Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Pluripotent stem cell
Cell type
iPS cells
NA
NA

Attributes by original data submitter

Sample

source_name
reprogramming cells
reprogramming stage
p24d+dox
chip antibody
H3K4me3

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to KAPA DNA Library Preparation Kits.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
14970420
Reads aligned (%)
90.7
Duplicates removed (%)
2.2
Number of peaks
26883 (qval < 1E-05)

hg19

Number of total reads
14970420
Reads aligned (%)
90.2
Duplicates removed (%)
2.2
Number of peaks
26623 (qval < 1E-05)

Base call quality data from DBCLS SRA