Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Pluripotent stem cell

Cell type

iPS cells

NA

NA

Sample

source_name

reprogramming cells

reprogramming stage

n24d+dox

chip antibody

H3K4me3

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to KAPA DNA Library Preparation Kits.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

11449735

Reads aligned (%)

54.2

Duplicates removed (%)

1.5

Number of peaks

7306 (qval < 1E-05)

hg19

Number of total reads

11449735

Reads aligned (%)

53.7

Duplicates removed (%)

1.5

Number of peaks

7333 (qval < 1E-05)