Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
shKDM2B stable cells, input
cell line
293T
stable shrna
shKDM2B
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA fragment was obtained from IP. 1ug DNA was used for the construction of sequencing libraries. The construction of library was performed using NEBNext®UltraTM DNA Library Prep Kit for Illumina (New England Biolabs, UK) according to the manufacturer's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
18305569
Reads aligned (%)
81.5
Duplicates removed (%)
2.8
Number of peaks
459 (qval < 1E-05)

hg19

Number of total reads
18305569
Reads aligned (%)
80.8
Duplicates removed (%)
2.8
Number of peaks
272 (qval < 1E-05)

Base call quality data from DBCLS SRA