GSM2357739: TE H3K27me3 rep1; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Blood
Cell type
CD4+ T cells
NA
NA
Attributes by original data submitter
Sample
source_name
KLRG1hiIL7Rlo terminal effector CD8 T cells
strain
C57BL/6
age
six to seven weeks
tissue
Spleen
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Splenocytes were isolated and specific CD8 T cell subsets were sorted by FACS ATAC-seq library was constructed by Nextera DNA Sample Prep Kit (Illumina). For ChIP-seq library, DNA was end-repaired using End-it End-repair kit (Epicentre) and then added an “A” base to the 3’ end of DNA fragments using Klenow (NEB). Then DNA was ligated with adaptors using quick DNA ligase (NEB) at 25°C for 15 min followed by size selection of 200-400 bp using AMPure SPRI beads (Beckman Coulter). The adaptor ligated DNA was amplified using NEBNext High-Fidelity 2X PCR master mix (NEB).