Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TRF2

Cell type

Cell type Class
Muscle
Cell type
Myotube
NA
NA

Attributes by original data submitter

Sample

source_name
Post-mitotic differentiated muscle cells - MYOTUBES
cell type
Post-mitotic differentiated muscle cells (myotubes)
origin
Muscle biopsy - deltoid
viral transduction
pWPIR-IRES-GFP
chip antibody
TRF2 (IMGENEX, monoclonal mouse IMG-124A, clone 4A794.15)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and TRF2-DNA complexes were isolated with antibody. Libraries were prepared according to the standards Illumina's instructions

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
72807440
Reads aligned (%)
94.6
Duplicates removed (%)
42.6
Number of peaks
1333 (qval < 1E-05)

hg19

Number of total reads
72807440
Reads aligned (%)
93.7
Duplicates removed (%)
44.2
Number of peaks
1751 (qval < 1E-05)

Base call quality data from DBCLS SRA