Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
B cells
NA
NA

Attributes by original data submitter

Sample

source_name
Cll cells
patient number
p4
patient age
75
patient sex
Male
disease level
Binet B
chip ab
Input
activation
CD74
cell type
B cells

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated cells and protein-DNA complexes were isolated with antibody. In-house ChIP-Seq protocol, Libraries were evaluated by Qubit and TapeStation. Sequencing libraries were constructed with barcodes to allow multiplexing of y samples on one lane.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
64137400
Reads aligned (%)
98.7
Duplicates removed (%)
3.8
Number of peaks
1819 (qval < 1E-05)

hg19

Number of total reads
64137400
Reads aligned (%)
97.8
Duplicates removed (%)
5.4
Number of peaks
1490 (qval < 1E-05)

Base call quality data from DBCLS SRA