Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
B cells
NA
NA

Attributes by original data submitter

Sample

source_name
Cll cells
diagnosis
CLL
patient number
p7
patient age
61
patient sex
Male
disease level
Binet B
chip ab
H3K27Ac (abcam ab4729)
activation
CD74
cell type
B cells

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated cells and protein-DNA complexes were isolated with antibody. In-house ChIP-Seq protocol, Libraries were evaluated by Qubit and TapeStation. Sequencing libraries were constructed with barcodes to allow multiplexing of y samples on one lane.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
41457653
Reads aligned (%)
96.5
Duplicates removed (%)
7.6
Number of peaks
4545 (qval < 1E-05)

hg19

Number of total reads
41457653
Reads aligned (%)
95.6
Duplicates removed (%)
9.0
Number of peaks
4420 (qval < 1E-05)

Base call quality data from DBCLS SRA