Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Blood

Cell type

B cells

NA

NA

Sample

source_name

Cll cells

diagnosis

CLL

patient number

p6

patient age

81

patient sex

Male

disease level

Binet B

chip ab

H3K4Me3 (Millipore, 07-473)

activation

IgG

cell type

B cells

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated cells and protein-DNA complexes were isolated with antibody. In-house ChIP-Seq protocol, Libraries were evaluated by Qubit and TapeStation. Sequencing libraries were constructed with barcodes to allow multiplexing of y samples on one lane.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

22250236

Reads aligned (%)

95.6

Duplicates removed (%)

4.4

Number of peaks

12783 (qval < 1E-05)

hg19

Number of total reads

22250236

Reads aligned (%)

94.6

Duplicates removed (%)

5.4

Number of peaks

12824 (qval < 1E-05)