Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

B cells

NA

NA

Sample

source_name

Cll cells

diagnosis

CLL

patient number

p3

patient age

57

patient sex

Female

disease level

Binet A

chip ab

Input

activation

IgG

cell type

B cells

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated cells and protein-DNA complexes were isolated with antibody. In-house ChIP-Seq protocol, Libraries were evaluated by Qubit and TapeStation. Sequencing libraries were constructed with barcodes to allow multiplexing of y samples on one lane.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

23060533

Reads aligned (%)

98.0

Duplicates removed (%)

4.5

Number of peaks

1103 (qval < 1E-05)

hg19

Number of total reads

23060533

Reads aligned (%)

97.0

Duplicates removed (%)

6.3

Number of peaks

1088 (qval < 1E-05)