Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
B cells
NA
NA

Attributes by original data submitter

Sample

source_name
Cll cells
diagnosis
CLL
patient number
p4
patient age
75
patient sex
Male
disease level
Binet B
chip ab
Input
activation
CD74
cell type
B cells

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated cells and protein-DNA complexes were isolated with antibody. In-house ChIP-Seq protocol, Libraries were evaluated by Qubit and TapeStation. Sequencing libraries were constructed with barcodes to allow multiplexing of y samples on one lane.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
26714617
Reads aligned (%)
98.1
Duplicates removed (%)
2.3
Number of peaks
957 (qval < 1E-05)

hg19

Number of total reads
26714617
Reads aligned (%)
97.2
Duplicates removed (%)
3.5
Number of peaks
885 (qval < 1E-05)

Base call quality data from DBCLS SRA