Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CD74

Cell type

Cell type Class
Blood
Cell type
B cells
NA
NA

Attributes by original data submitter

Sample

source_name
Cll cells
diagnosis
CLL
patient number
p2
patient age
70
patient sex
Male
disease level
Binet B
chip ab
CD74 (BD Bioscience 555317)
activation
CD74
cell type
B cells

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated cells and protein-DNA complexes were isolated with antibody. In-house ChIP-Seq protocol, Libraries were evaluated by Qubit and TapeStation. Sequencing libraries were constructed with barcodes to allow multiplexing of y samples on one lane.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
11743129
Reads aligned (%)
75.7
Duplicates removed (%)
11.1
Number of peaks
595 (qval < 1E-05)

hg38

Number of total reads
11743129
Reads aligned (%)
77.4
Duplicates removed (%)
10.1
Number of peaks
546 (qval < 1E-05)

Base call quality data from DBCLS SRA