Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CD74

Cell type

Cell type Class
Blood
Cell type
B cells
NA
NA

Attributes by original data submitter

Sample

source_name
Cll cells
diagnosis
CLL
patient number
p1
patient age
71
patient sex
Male
disease level
Binet B
chip ab
CD74 (BD Bioscience 555317)
activation
CD74
cell type
B cells

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated cells and protein-DNA complexes were isolated with antibody. In-house ChIP-Seq protocol, Libraries were evaluated by Qubit and TapeStation. Sequencing libraries were constructed with barcodes to allow multiplexing of y samples on one lane.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
20022063
Reads aligned (%)
73.3
Duplicates removed (%)
17.9
Number of peaks
960 (qval < 1E-05)

hg38

Number of total reads
20022063
Reads aligned (%)
75.1
Duplicates removed (%)
16.7
Number of peaks
957 (qval < 1E-05)

Base call quality data from DBCLS SRA