Antigen

Antigen Class

TFs and others

Antigen

CD74

Cell type

Cell type Class

Blood

Cell type

B cells

NA

NA

Sample

source_name

Cll cells

diagnosis

CLL

patient number

p1

patient age

71

patient sex

Male

disease level

Binet B

chip ab

CD74 (BD Bioscience 555317)

activation

CD74

cell type

B cells

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated cells and protein-DNA complexes were isolated with antibody. In-house ChIP-Seq protocol, Libraries were evaluated by Qubit and TapeStation. Sequencing libraries were constructed with barcodes to allow multiplexing of y samples on one lane.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

20022063

Reads aligned (%)

90.2

Duplicates removed (%)

16.7

Number of peaks

957 (qval < 1E-05)

hg19

Number of total reads

20022063

Reads aligned (%)

89.3

Duplicates removed (%)

17.9

Number of peaks

960 (qval < 1E-05)