Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
Leukemic bone marrow
NA
NA

Attributes by original data submitter

Sample

source_name
leukemic bone marrow cells
strain
Gfi1b(fl/fl) x Nup98(tg) x MxCre(wt)
tissue
bone marrow
cell type
leukemic bone marrow cells
chip-antibody
rabbit-IgG, Sigma, I5006

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Bone marrow (BM) cells were isolated from Gfi1bfl/fl MxCretg NUP98/HOXD13tg and Gfi1bfl/fl MxCrewt Nup98/HOXD13 mice. BM cells were homogenised and ery lysis was performed. 1x10^7 cells were fixed with 1% formaldehyde and sonicated for 20 min. Histone-DNA antibody were isolated with antibody Libraries were prepared using the NEBNext® UltraTM DNA Library Prep Kit for Illumina®. Samples were pooled after quality check.

Sequencing Platform

instrument_model
Illumina Genome Analyzer

mm10

Number of total reads
8692355
Reads aligned (%)
93.2
Duplicates removed (%)
56.7
Number of peaks
79 (qval < 1E-05)

mm9

Number of total reads
8692355
Reads aligned (%)
93.1
Duplicates removed (%)
56.9
Number of peaks
90 (qval < 1E-05)

Base call quality data from DBCLS SRA