Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Rorc

Cell type

Cell type Class
Blood
Cell type
Thymocytes
MeSH Description
HEMATOPOIETIC PROGENITOR CELLS that have migrated to the THYMUS where they differentiate into T-LYMPHOCYTES. Thymocytes are classified into maturational stages based on the expression of CELL SURFACE ANTIGENS.

Attributes by original data submitter

Sample

source_name
thymocytes
chip antibody
B2D and AFKJS-9 from eBioscience
strain
C57BL/6

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Library construction and sequencing were performed using the standard Active Motif ChIP-seq protocol.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
38741976
Reads aligned (%)
90.2
Duplicates removed (%)
65.8
Number of peaks
747 (qval < 1E-05)

mm9

Number of total reads
38741976
Reads aligned (%)
90.1
Duplicates removed (%)
65.8
Number of peaks
730 (qval < 1E-05)

Base call quality data from DBCLS SRA