Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Blood
Cell type
MV-4-11
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
MV4;11 cell line
cell line
MV4;11
tissue
peripheral blood
clickable molecule
IBET–TCO
competitor molecule
IBET

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysate were isolated from formaldehybe cross-linked cells and digested with micrococcal nuclease to mononucleosomes, then incubated with the clickable molecule with/without competitor. Chem-precipitation was performed with pre-clicked biotin-tetrazine conjugated beads NEBNext ChIP-seq library prep master mix set

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
30917475
Reads aligned (%)
98.7
Duplicates removed (%)
17.8
Number of peaks
584 (qval < 1E-05)

hg19

Number of total reads
30917475
Reads aligned (%)
97.9
Duplicates removed (%)
20.0
Number of peaks
642 (qval < 1E-05)

Base call quality data from DBCLS SRA