Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Blood

Cell type

MV-4-11

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

MV4;11 cell line

cell line

MV4;11

tissue

peripheral blood

clickable molecule

IBET–TCO

competitor molecule

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysate were isolated from formaldehybe cross-linked cells and digested with micrococcal nuclease to mononucleosomes, then incubated with the clickable molecule with/without competitor. Chem-precipitation was performed with pre-clicked biotin-tetrazine conjugated beads NEBNext ChIP-seq library prep master mix set

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

33806087

Reads aligned (%)

98.9

Duplicates removed (%)

9.4

Number of peaks

2974 (qval < 1E-05)

hg19

Number of total reads

33806087

Reads aligned (%)

98.1

Duplicates removed (%)

11.5

Number of peaks

2823 (qval < 1E-05)