Antigen

Antigen Class

TFs and others

Antigen

BRD4

Cell type

Cell type Class

Blood

Cell type

MV-4-11

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

MV411_BRD4

cell line

MV4;11

tissue source

peripheral blood

cell line

MV4;11

chip antibody

anti-BRD4 (E2A7X, Cell Signalling)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysate were isolated from formaldehybe cross-linked cells and digested with micrococcal nuclease to mononucleosomes, then immunoprecipitated with antibody NEBNext ChIP-seq library prep master mix set

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

41740673

Reads aligned (%)

97.7

Duplicates removed (%)

12.6

Number of peaks

1081 (qval < 1E-05)

hg19

Number of total reads

41740673

Reads aligned (%)

96.8

Duplicates removed (%)

15.0

Number of peaks

980 (qval < 1E-05)