Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRD4

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562_BRD4
cell line
K562
tissue source
bone marrow
cell line
K562
chip antibody
anti-BRD4 (E2A7X, Cell Signalling)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysate were isolated from formaldehybe cross-linked cells and digested with micrococcal nuclease to mononucleosomes, then immunoprecipitated with antibody NEBNext ChIP-seq library prep master mix set

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
42902512
Reads aligned (%)
90.6
Duplicates removed (%)
7.8
Number of peaks
582 (qval < 1E-05)

hg38

Number of total reads
42902512
Reads aligned (%)
93.4
Duplicates removed (%)
5.8
Number of peaks
613 (qval < 1E-05)

Base call quality data from DBCLS SRA