GSM1922950: NICD GSC8 12d-das ChIPseq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
NOTCH1
Cell type
Cell type Class
Neural
Cell type
GSC8
NA
NA
Attributes by original data submitter
Sample
source_name
NICD_GSC8_12d-das
cell line
Patient-derived neurosphere line GSC8
drug treatment
12 day 1 µM dasatinib
chip-antibody
cleaved Notch1 (Val1744) (CST, 4147, lot 4)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells was fixed in 1% formaldehyde and snap frozen in liquid nitrogen and stored at -80ºC for at least overnight. Sonication of crosslinked cells was calibrated such that DNA was sheared to between 400bp and 2000bp. ChIP DNA was used to generate sequencing libraries by end repair (End-It DNA repair kit, Epicentre), 3’ A base overhang addition via Klenow fragment (NEB), and ligation of barcoded sequencing adapters.