Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Neural
Cell type
GSC8
NA
NA

Attributes by original data submitter

Sample

source_name
Input_GSC8Per
cell line
Patient-derived neurosphere line GSC8
drug treatment
chronic 1 µM dasatinib
chip-antibody
NA

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells was fixed in 1% formaldehyde and snap frozen in liquid nitrogen and stored at -80ºC for at least overnight. Sonication of crosslinked cells was calibrated such that DNA was sheared to between 400bp and 2000bp. ChIP DNA was used to generate sequencing libraries by end repair (End-It DNA repair kit, Epicentre), 3’ A base overhang addition via Klenow fragment (NEB), and ligation of barcoded sequencing adapters.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
30206748
Reads aligned (%)
96.4
Duplicates removed (%)
0.0
Number of peaks
141 (qval < 1E-05)

hg19

Number of total reads
30206748
Reads aligned (%)
96.4
Duplicates removed (%)
0.1
Number of peaks
302 (qval < 1E-05)

Base call quality data from DBCLS SRA