Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
PAX6

Cell type

Cell type Class
Pancreas
Cell type
EndoC-BH1
NA
NA

Attributes by original data submitter

Sample

source_name
EndoC cells
tissue
human beta cell line EndoC
age
--
genotype
WT
chip antibody
Rabbit anti-Pax6 (Biolegend, 901301)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-Seq: Sonicated chromatin from Min6 cells or EndoC cells was immunoprecipitated with rabbit anti Pax6 (Biolegend) and that of sorted pancreatic beta cells using anti-H3K9Ac antibody. ChIP-Seq: Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Prep Kit.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
39415272
Reads aligned (%)
62.8
Duplicates removed (%)
29.0
Number of peaks
2340 (qval < 1E-05)

hg38

Number of total reads
39415272
Reads aligned (%)
64.4
Duplicates removed (%)
27.9
Number of peaks
2555 (qval < 1E-05)

Base call quality data from DBCLS SRA