Curated Sample Data


Genome
dm3
Antigen Class
Input control
Antigen
Input control
Cell type Class
Cell line
Cell type
S2

Cell type information


Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by Original Data Submitter


source_name
S2_2xlink IgG ChIP-seq
cell line
S2 cells
chip antibody
Normal rabbit IgG
chip antibody information
Cell Signaling, catalog number 2729
crosslinker
2mM ethylene glycol bis(succinimidyl succinate) and 1% formaldehyde

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were sonicated and cleared of cellular debris. Lysates were immunoprecipitated with the corresponding antibodies Briefly, DNA ends were repaired with T4 DNA polymerase, Klenow Large Fragment DNA polyermerase and T4 polynclease kinase. DNA was purified with PEG bead slurry. Illumina short sequencing adaptor was ligated to DNA overnight. Library was purified with PEG beads

Platform Information


instrument_model
Illumina MiSeq

External Database Query

Logs in read processing pipeline


Number of total reads
3010819
Reads aligned (%)
58.5
Duplicates removed (%)
1.4
Number of peaks
841 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA