Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Gug

Cell type

Cell type Class
Cell line
Cell type
S2
Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by original data submitter

Sample

source_name
S2_2xlink Atro ChIP-seq
cell line
S2 cells
chip antibody
Rabbit anti-Atro sera (SG2524)
chip antibody information
raised in rabbit, against Atro (amino acids 121-134)
crosslinker
2mM ethylene glycol bis(succinimidyl succinate) and 1% formaldehyde

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were sonicated and cleared of cellular debris. Lysates were immunoprecipitated with the corresponding antibodies Briefly, DNA ends were repaired with T4 DNA polymerase, Klenow Large Fragment DNA polyermerase and T4 polynclease kinase. DNA was purified with PEG bead slurry. Illumina short sequencing adaptor was ligated to DNA overnight. Library was purified with PEG beads

Sequencing Platform

instrument_model
Illumina MiSeq

dm3

Number of total reads
3638709
Reads aligned (%)
163.4
Duplicates removed (%)
1.8
Number of peaks
3338 (qval < 1E-05)

dm6

Number of total reads
3638709
Reads aligned (%)
161.1
Duplicates removed (%)
2.8
Number of peaks
2681 (qval < 1E-05)

Base call quality data from DBCLS SRA