raised in rabbit, against Atro (amino acids 121-134)
crosslinker
2mM ethylene glycol bis(succinimidyl succinate) and 1% formaldehyde
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were sonicated and cleared of cellular debris. Lysates were immunoprecipitated with the corresponding antibodies Briefly, DNA ends were repaired with T4 DNA polymerase, Klenow Large Fragment DNA polyermerase and T4 polynclease kinase. DNA was purified with PEG bead slurry. Illumina short sequencing adaptor was ligated to DNA overnight. Library was purified with PEG beads