Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Bach2

Cell type

Cell type Class
Blood
Cell type
Pro-B cells
NA
NA

Attributes by original data submitter

Sample

source_name
pre-pro B cells
strain
EBF-/- C57BL/6
cell type
progenitor cells
chip antibody
Bach2 (Bach2N2)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Ebf1 deficient cells were fixed 1% formaldehyde, followed by quenching with 125mM of glycine. The cells were washed with ice-cold PBS and were lysed. The crosslinked chromatin was shared with a Bioruptor Sonicator (CosmoBio) and target protein-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared from 1~3 ng of ChIP DNA with the use of Ovation Ultralow DR Multiplex System (Nugen). The DNA libraries were clonally amplified on a flow cell and sequenced on HiSeq2500 (HiSeq Control Software v2.2.38, Illumina) with 51-mer paired-end sequence. Image analysis and base calling were performed using Real-Time Analysis Software (v1.18.61, Illumina).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
84409726
Reads aligned (%)
97.0
Duplicates removed (%)
60.9
Number of peaks
23936 (qval < 1E-05)

mm9

Number of total reads
84409726
Reads aligned (%)
96.9
Duplicates removed (%)
61.3
Number of peaks
23900 (qval < 1E-05)

Base call quality data from DBCLS SRA