Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Others
Cell type
Olfactory epithelium
NA
NA

Attributes by original data submitter

Sample

source_name
olfactory bulb
strain
CD1
age
postnatal day 0-2
tissue
olfactory bulb
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The lysate was sheared into 200- to 1000-bp fragments and Sp9-DNA complex was isolated with specific Sp9 antibody. The yield of ChIP DNA was determined via Quant IT fluorescence assay (Life Technologies) and enrichment efficiencies of ChIP reaction were evaluated by qPCR. Illumina sequencing libraries were generated with NEBNext® Ultra™ DNA Library Prep Kit (New England Biolabs) by following the manufacturer's manual. The library quality was determined by using Agilent 2100 Bioanalyzer(Agilent), and then, subjected to high-throughput 150 base paired-end sequencing on Illumina Hiseq sequencer according to the manufacturer’s recommended protocol.

Sequencing Platform

instrument_model
Illumina HiSeq 4000

mm10

Number of total reads
11039411
Reads aligned (%)
77.2
Duplicates removed (%)
9.8
Number of peaks
93 (qval < 1E-05)

mm9

Number of total reads
11039411
Reads aligned (%)
77.2
Duplicates removed (%)
9.7
Number of peaks
73 (qval < 1E-05)

Base call quality data from DBCLS SRA