Antigen

Antigen Class

TFs and others

Antigen

BRCA1

Cell type

Cell type Class

Bone

Cell type

EWS502

NA

NA

Sample

source_name

Ewing's sarcoma EWS502

cell line

EWS502

cell type

Ewing's sarcoma

treatment agent

0.25μM Etoposide for 6 hours

chip antibody

BRCA1 (Santa Cruz, Cat# sc-646)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Genomic DNA was extracted from sub-confluent dishes, fixed and sheared to an average length of 200-1500bp. 10% of the samples from each cell line were pooled to form the Input. RNAPII/BRCA1 antibody was used for immunoprecipitation. ChIP DNA was purified, sheared further to an average length of 350 bp and sequenced after library construction. Sequencing library was prepared using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 3000

hg38

Number of total reads

39980004

Reads aligned (%)

99.8

Duplicates removed (%)

4.5

Number of peaks

964 (qval < 1E-05)

hg19

Number of total reads

39980004

Reads aligned (%)

99.8

Duplicates removed (%)

4.8

Number of peaks

977 (qval < 1E-05)