GSM2325726: C3 deficient ChIP-seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
CASP3
Cell type
Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
MCF-7 cells
cell line
MCF-7
modification
Stably transfected with empty vector
tissue
Breast
treatment
No treatment
antibody
Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb from Cell Signaling, Ref: #9664, Batch: 0019
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Crosslinking, extraction and shearing of chromatin was performed by using the Truchip chromatin shearing reagent kit from 1,500,000 cells by following manufacturer protocol. Then, sheared chromatin was immunoprecipitated with different antibodies (anti-active Caspase-3 or control Immunoglobulin) by using the Active Motif's ChIP IT Kit by following manufacturer's instructions Libraries were prepared according to NEB's instructions accompanying the NEBNext Ultra DNA Library Prep Kit for Illumina. After library validation on a fragment analyzer, the purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the MiSeq device following the manufacturer's protocols.