Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Neural
Cell type
AtT-20
Tissue
Pituitary
Disease
Tumor

Attributes by original data submitter

Sample

source_name
AtT-20 cells expressing 3x flag-Pax7
cell type
pituitary corticotroph cell
passages
10-20
cell line
AtT-20 cells
treatment
non treated
chip antibody
IgG Sigma I5381

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclei lysates were sonicated and protein-DNA complexes were isolated with specific antibodies. Fragments of 200-400bp were kept for library preparation Libraries were prepared according to Illumina's instructions. Libraries were sequenced on the Illumina Hi-seq 2000 following the manufacturer's protocols to obtain 50bp single end reads.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
101480328
Reads aligned (%)
44.6
Duplicates removed (%)
13.8
Number of peaks
497 (qval < 1E-05)

mm9

Number of total reads
101480328
Reads aligned (%)
89.4
Duplicates removed (%)
19.4
Number of peaks
1123 (qval < 1E-05)

Base call quality data from DBCLS SRA