Antigen

Antigen Class

Histone

Antigen

H3K9K14ac

Cell type

Cell type Class

Pluripotent stem cell

Cell type

iPS cells

NA

NA

Sample

source_name

fibroblast-derived iPSCs fMG03

strain

C57BL/6

cell type

derived iPSCs fMG03

chip antibody

H3K9-14Ac(Diagenode,C15410200)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP with iDeal ChIP-seq kit (Diagenode # C01010051), MinElute PCR-purification kit (QIAGEN #28006), quantified using the Quant-iT PicoGreen ds DNA assay (Life Technologies #Q33120) Libraries were prepared from 5-10 ng DNA using the NEBNext ChIP-Seq Library Prep Reagent Set for Illumina with NEBNext High-Fidelity 2x PCR Master Mix according to the manufacturer’s instructions (New England Biolabs, Ipswich, MA) with the following modifications: A 1:1 Ampure cleanup was added after adaptor ligation a total of 2 times.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

46393067

Reads aligned (%)

98.4

Duplicates removed (%)

5.4

Number of peaks

29933 (qval < 1E-05)

mm9

Number of total reads

46393067

Reads aligned (%)

98.3

Duplicates removed (%)

5.4

Number of peaks

29914 (qval < 1E-05)