Curated Sample Data


Genome
dm3
Antigen Class
Input control
Antigen
Input control
Cell type Class
Cell line
Cell type
S2

Cell type information


Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by Original Data Submitter


source_name
Drosophila S2 cells, WT, input
cell line
S2
genotype/variation
WT
chip antibody
none

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was conducted on 1 x 10^7 cell equivalents of chromatin from Drosophila S2 cells. For input controls, 1 x 10^6 cell equivalents of chromatin was used. Soluble chromatin was incubated with rabbit α-MLF (gift from Dr. Anne Plessis), rat α-MLF (this work), rabbit α-DnaJ-1 (this work). ChIP-seq libraries were prepared using ChIP-Seq DNA Sample Prep Kit (Illumina) or KAPA High Throughput Library Preparation Kit, Roche.

Platform Information


instrument_model
Illumina HiSeq 2000

External Database Query

Logs in read processing pipeline


Number of total reads
25521864
Reads aligned (%)
95.9
Duplicates removed (%)
12.6
Number of peaks
3728 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA