Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Mlf

Cell type

Cell type Class
Cell line
Cell type
S2
Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by original data submitter

Sample

source_name
Drosophila S2 cells, WT, MLF ChIP
cell line
S2
genotype/variation
WT
chip antibody
MLF

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was conducted on 1 x 10^7 cell equivalents of chromatin from Drosophila S2 cells. For input controls, 1 x 10^6 cell equivalents of chromatin was used. Soluble chromatin was incubated with rabbit α-MLF (gift from Dr. Anne Plessis), rat α-MLF (this work), rabbit α-DnaJ-1 (this work). ChIP-seq libraries were prepared using ChIP-Seq DNA Sample Prep Kit (Illumina) or KAPA High Throughput Library Preparation Kit, Roche.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm3

Number of total reads
34893030
Reads aligned (%)
72.9
Duplicates removed (%)
23.8
Number of peaks
14841 (qval < 1E-05)

dm6

Number of total reads
34893030
Reads aligned (%)
70.8
Duplicates removed (%)
25.7
Number of peaks
14424 (qval < 1E-05)

Base call quality data from DBCLS SRA