GSM2311178: tub uBcd Bcd ChIP B; Drosophila melanogaster; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
bcd
Cell type
Cell type Class
Embryo
Cell type
Embryos
NA
NA
Attributes by original data submitter
Sample
source_name
uniform Bcd (high) Chromatin Immunoprecipitation
timepoint
NC14
tissue
embryo
number of embryos
200
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Embryos from a 0-4 hour collection were crosslinked in formaldehyde for 15 minutes prior to quenching with glycine and 3 1x PBS washes. Embryos were hand sorted under a dissecting scope in order to collect NC14 stage only. Collected embryos were frozen at -80° until sufficient numbers were collected. To perform ChIP, collected embryos were thawed and homogenized prior to sonication in RIPA buffer. Sheared chromatin was subjected to overnight immunoprecipitation with an anti-GFP antibody and immunocomplexes were precipitated with protein-G dynambeads. Pellets were washed extensively prior to elution and crosslink reversal. ChIP samples were subjected to library preparation using the NEB Next Library Mastermix Kit following the manufacturer's instructions.