Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SPDEF

Cell type

Cell type Class
Lung
Cell type
A549
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
A549_SPDEF_ChIPseq
cell line background
A549
cell type
lung carcinoma cells
infected with
lentivirus expressing NKX2-1
chip antibody
SPDEF antibody
chip antibody vendor
Santa Cruz Biotechnology (Santa Cruz, CA)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed as described in Maeda et al., J Clin Invest. (2012) doi: 10.1172/JCI64048 Illumina sequencing libraries were prepared as described in Maeda et al., J Clin Invest. (2012) doi: 10.1172/JCI64048

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
13054736
Reads aligned (%)
88.0
Duplicates removed (%)
9.9
Number of peaks
1106 (qval < 1E-05)

hg38

Number of total reads
13054736
Reads aligned (%)
89.6
Duplicates removed (%)
9.2
Number of peaks
1110 (qval < 1E-05)

Base call quality data from DBCLS SRA