Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NKX2-1

Cell type

Cell type Class
Lung
Cell type
A549
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
A549_NKX2-1_ChIPseq
cell line background
A549
cell type
lung carcinoma cells
infected with
lentivirus expressing NKX2-1
chip antibody
NKX2-1 (TTF-1) antibody
chip antibody vendor
Seven Hills Bioreagents (Cincinnati, OH)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed as described in Maeda et al., J Clin Invest. (2012) doi: 10.1172/JCI64048 Illumina sequencing libraries were prepared as described in Maeda et al., J Clin Invest. (2012) doi: 10.1172/JCI64048

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
5434611
Reads aligned (%)
187.8
Duplicates removed (%)
4.0
Number of peaks
2895 (qval < 1E-05)

hg38

Number of total reads
5434611
Reads aligned (%)
190.0
Duplicates removed (%)
3.9
Number of peaks
2980 (qval < 1E-05)

Base call quality data from DBCLS SRA