Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Larvae
Cell type
L4
NA
NA

Attributes by original data submitter

Sample

source_name
whole organism
developmental stage
L4
strain
wild type Bristol N2
chip antibody
H3K4me3 (Abcam ab8580)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed with a protocol modified from that of Kolasinska-Zwierz et al., 2009. Lysates were clarified from sonicated chromatin and histone-DNA complexes were isolated with antibody. Libraries were prepared using the ChIP-seq DNA sample prep kit (Illumina) with a protocol adapted from Meyer and Kircher, 2010.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

ce11

Number of total reads
41298351
Reads aligned (%)
76.3
Duplicates removed (%)
46.7
Number of peaks
5071 (qval < 1E-05)

ce10

Number of total reads
41298351
Reads aligned (%)
76.3
Duplicates removed (%)
46.7
Number of peaks
5067 (qval < 1E-05)

Base call quality data from DBCLS SRA