Curated Sample Data


Genome
ce10
Antigen Class
Histone
Antigen
H3K4me3
Cell type Class
Larvae
Cell type
L4

Cell type information


NA
NA

Attributes by Original Data Submitter


source_name
whole organism
developmental stage
L4
strain
wild type Bristol N2
chip antibody
H3K4me3 (Abcam ab8580)

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed with a protocol modified from that of Kolasinska-Zwierz et al., 2009. Lysates were clarified from sonicated chromatin and histone-DNA complexes were isolated with antibody. Libraries were prepared using the ChIP-seq DNA sample prep kit (Illumina) with a protocol adapted from Meyer and Kircher, 2010.

Platform Information


instrument_model
Illumina HiSeq 2000

External Database Query

Logs in read processing pipeline


Number of total reads
41298351
Reads aligned (%)
76.3
Duplicates removed (%)
46.7
Number of peaks
5067 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA