Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Irf4

Cell type

Cell type Class
Blood
Cell type
Pre-B cells
NA
NA

Attributes by original data submitter

Sample

source_name
stromal adherent WT pre-B cell progenitors derived from WT mice
cell type
stromal adherent pre-B cell progenitors
genotype
WT
passages
3 to 8
antibody
Santa Cruz Biotech/sc-6059x

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed according to previously described protocols (Zhang et al., NI 2011) and detailed in Hu et al., G&D 2016 Immunoprecipitated (ChIP) DNA and Input DNA were used to prepare sequencing libraries according to a modified version of the Illumina/Solexa Genomic DNA protocol and is detailed in Hu et al., G&D 2016.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
28564212
Reads aligned (%)
96.9
Duplicates removed (%)
11.6
Number of peaks
63634 (qval < 1E-05)

mm9

Number of total reads
28564212
Reads aligned (%)
96.7
Duplicates removed (%)
11.6
Number of peaks
63439 (qval < 1E-05)

Base call quality data from DBCLS SRA