Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

Pre-B cells

NA

NA

Sample

source_name

stromal adherent IKDN pre-B cell progenitors derived from IKDN CD2 Cre mice

cell type

stromal adherent pre-B cell progenitors

genotype

IKDN CD2 Cre

passages

3 to 8

antibody

none (input)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP was performed according to previously described protocols (Zhang et al., NI 2011) and detailed in Hu et al., G&D 2016 Immunoprecipitated (ChIP) DNA and Input DNA were used to prepare sequencing libraries according to a modified version of the Illumina/Solexa Genomic DNA protocol and is detailed in Hu et al., G&D 2016.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

18465088

Reads aligned (%)

98.2

Duplicates removed (%)

10.5

Number of peaks

364 (qval < 1E-05)

mm9

Number of total reads

18465088

Reads aligned (%)

98.0

Duplicates removed (%)

10.6

Number of peaks

393 (qval < 1E-05)