Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Ebf1

Cell type

Cell type Class
Blood
Cell type
Pre-B cells
NA
NA

Attributes by original data submitter

Sample

source_name
stromal adherent IKDN pre-B cell progenitors derived from IKDN CD2 Cre mice
cell type
stromal adherent pre-B cell progenitors
genotype
IKDN CD2 Cre
passages
3 to 8
antibody
Abnova/H00001879-M01

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed according to previously described protocols (Zhang et al., NI 2011) and detailed in Hu et al., G&D 2016 Immunoprecipitated (ChIP) DNA and Input DNA were used to prepare sequencing libraries according to a modified version of the Illumina/Solexa Genomic DNA protocol and is detailed in Hu et al., G&D 2016.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
20587644
Reads aligned (%)
92.8
Duplicates removed (%)
11.4
Number of peaks
18670 (qval < 1E-05)

mm9

Number of total reads
20587644
Reads aligned (%)
92.6
Duplicates removed (%)
11.5
Number of peaks
18687 (qval < 1E-05)

Base call quality data from DBCLS SRA