Antigen

Antigen Class

TFs and others

Antigen

Stat5a

Cell type

Cell type Class

Blood

Cell type

Leukemia, B-Cell

MeSH Description

A malignant disease of the B-LYMPHOCYTES in the bone marrow and/or blood.

Sample

source_name

ChIP-Seq_STAT5

strain background

C57BL/6

genotype/variation

Stat5b-CAxBlnk+/- leukemic mouse

tissue

Lymph nodes

cell type

B cell progenitor leukemia

chip antibody

anti-STAT5 antibody (Santa Cruz, sc-835 (C-17))

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lymph nodes from Stat5b-CAxBlnk+/- leukemic mouse were made into single cell suspensions, stimulated with 10 ng/mL murine IL7 for 30 minutes at 37°C, and fixed with 1% formaldyde at room temperature for 10 minutes. Whole cell extracts were sonicated with Diagenode Bioruptor to solubilize and fragment the chromatin to an average size of 200 bp. The chromatin was then immunoprecipitated with the Santa Cruz sc-835 (C-17)x anti-STAT5 antibody. Followed the Illumina TruSeq ChIP sample kit

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

61192913

Reads aligned (%)

19.7

Duplicates removed (%)

9.6

Number of peaks

2524 (qval < 1E-05)

mm9

Number of total reads

61192913

Reads aligned (%)

19.7

Duplicates removed (%)

9.6

Number of peaks

2518 (qval < 1E-05)