Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Embryo

Cell type

Embryonic limb

NA

NA

Sample

age

10.5 day

dev stage

embryonic

donor ID

ENCDO956IXV

tissue

limb

sex

pooled male and female

sample type

tissue

lab

Len Pennacchio, LBNL

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

general protocol: https://www.encodeproject.org/documents/18580e80-0907-4258-a412-46bcc37bd040/@@download/attachment/Ren%20Lab%20ENCODE%20Chromatin%20Immunoprecipitation%20Protocol%20MicroChIP.pdf general protocol: https://www.encodeproject.org/documents/1fcaab50-6ca0-4778-88cb-5f6b85170d21/@@download/attachment/Ren%20Lab%20ENCODE%20Tissue%20Fixation%20and%20Sonication%20Protocol%20MicroChIP.pdf

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

151349105

Reads aligned (%)

91.8

Duplicates removed (%)

65.6

Number of peaks

2145 (qval < 1E-05)

mm9

Number of total reads

151349105

Reads aligned (%)

91.7

Duplicates removed (%)

65.7

Number of peaks

2168 (qval < 1E-05)