Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MYC

Cell type

Cell type Class
Neural
Cell type
BT168
NA
NA

Attributes by original data submitter

Sample

source_name
BT168FO (Glioblastoma stem cells)
cell line
BT168 glioblastoma stem cells (De Bacco et al., Cancer Res. 2012) with Dox inducible FLAG-Omomyc
treatment
Dox treated
chip antibody
c-Myc N-262 rabbit polyclonal antibody (sc-764X, Santa Cruz Biotech)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
U87FO cells were processed according to Myers Lab ChIP-seq Protocol v041610.2. BT168FO cells were processed through the MAGnify Chromatin Immunoprecipitation System (Thermo Fisher). Libraries were prepared at the Istituto di Genomica Applicata (IGA) in Udine (http://www.appliedgenomics.org/) according to Illumina TruSeq DNA Sample Preparation Guide

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
41931489
Reads aligned (%)
81.9
Duplicates removed (%)
14.2
Number of peaks
1100 (qval < 1E-05)

hg38

Number of total reads
41931489
Reads aligned (%)
84.0
Duplicates removed (%)
12.9
Number of peaks
1316 (qval < 1E-05)

Base call quality data from DBCLS SRA