Antigen

Antigen Class

TFs and others

Antigen

MYC

Cell type

Cell type Class

Neural

Cell type

BT168

NA

NA

Sample

source_name

BT168FO (Glioblastoma stem cells)

cell line

BT168 glioblastoma stem cells (De Bacco et al., Cancer Res. 2012) with Dox inducible FLAG-Omomyc

treatment

Dox treated

chip antibody

c-Myc N-262 rabbit polyclonal antibody (sc-764X, Santa Cruz Biotech)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

U87FO cells were processed according to Myers Lab ChIP-seq Protocol v041610.2. BT168FO cells were processed through the MAGnify Chromatin Immunoprecipitation System (Thermo Fisher). Libraries were prepared at the Istituto di Genomica Applicata (IGA) in Udine (http://www.appliedgenomics.org/) according to Illumina TruSeq DNA Sample Preparation Guide

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg19

Number of total reads

41931489

Reads aligned (%)

95.5

Duplicates removed (%)

14.2

Number of peaks

1100 (qval < 1E-05)

hg38

Number of total reads

41931489

Reads aligned (%)

96.4

Duplicates removed (%)

12.9

Number of peaks

1316 (qval < 1E-05)