Antigen

Antigen Class

TFs and others

Antigen

MYC

Cell type

Cell type Class

Neural

Cell type

U-87 MG

Primary Tissue

Brain

Tissue Diagnosis

Glioblastoma

Sample

source_name

U87FO (Glioblastoma cell line)

cell line

U-87 MG (ATCC HTB-14) cells with Dox inducible FLAG-Omomyc

treatment

Dox treated

chip antibody

c-Myc N-262 rabbit polyclonal antibody (sc-764X, Santa Cruz Biotech)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

U87FO cells were processed according to Myers Lab ChIP-seq Protocol v041610.2. BT168FO cells were processed through the MAGnify Chromatin Immunoprecipitation System (Thermo Fisher). Libraries were prepared at the Istituto di Genomica Applicata (IGA) in Udine (http://www.appliedgenomics.org/) according to Illumina TruSeq DNA Sample Preparation Guide

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

25988001

Reads aligned (%)

94.9

Duplicates removed (%)

38.7

Number of peaks

821 (qval < 1E-05)

hg19

Number of total reads

25988001

Reads aligned (%)

93.9

Duplicates removed (%)

40.0

Number of peaks

788 (qval < 1E-05)