Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MYC

Cell type

Cell type Class
Neural
Cell type
U-87 MG
Primary Tissue
Brain
Tissue Diagnosis
Glioblastoma

Attributes by original data submitter

Sample

source_name
U87FO (Glioblastoma cell line)
cell line
U-87 MG (ATCC HTB-14) cells with Dox inducible FLAG-Omomyc
treatment
Dox treated
chip antibody
c-Myc N-262 rabbit polyclonal antibody (sc-764X, Santa Cruz Biotech)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
U87FO cells were processed according to Myers Lab ChIP-seq Protocol v041610.2. BT168FO cells were processed through the MAGnify Chromatin Immunoprecipitation System (Thermo Fisher). Libraries were prepared at the Istituto di Genomica Applicata (IGA) in Udine (http://www.appliedgenomics.org/) according to Illumina TruSeq DNA Sample Preparation Guide

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
25988001
Reads aligned (%)
56.3
Duplicates removed (%)
40.0
Number of peaks
788 (qval < 1E-05)

hg38

Number of total reads
25988001
Reads aligned (%)
58.1
Duplicates removed (%)
38.7
Number of peaks
821 (qval < 1E-05)

Base call quality data from DBCLS SRA