Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Neural

Cell type

U-87 MG

Primary Tissue

Brain

Tissue Diagnosis

Glioblastoma

Sample

source_name

U87FO (Glioblastoma cell line)

cell line

U-87 MG (ATCC HTB-14) cells with Dox inducible FLAG-Omomyc

treatment

Untreated with Dox

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

U87FO cells were processed according to Myers Lab ChIP-seq Protocol v041610.2. BT168FO cells were processed through the MAGnify Chromatin Immunoprecipitation System (Thermo Fisher). Libraries were prepared at the Istituto di Genomica Applicata (IGA) in Udine (http://www.appliedgenomics.org/) according to Illumina TruSeq DNA Sample Preparation Guide

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

30856533

Reads aligned (%)

97.6

Duplicates removed (%)

7.7

Number of peaks

1017 (qval < 1E-05)

hg19

Number of total reads

30856533

Reads aligned (%)

96.6

Duplicates removed (%)

9.0

Number of peaks

951 (qval < 1E-05)