Antigen

Antigen Class

TFs and others

Antigen

Epitope tags

Cell type

Cell type Class

Neural

Cell type

BT168

NA

NA

Sample

source_name

BT168FO (Glioblastoma stem cells)

cell line

BT168 glioblastoma stem cells (De Bacco et al., Cancer Res. 2012) with Dox inducible FLAG-Omomyc

treatment

Dox treated

chip antibody

FLAG® M2 mouse monoclonal antibody (Sigma-Adlrich, F1804)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

U87FO cells were processed according to Myers Lab ChIP-seq Protocol v041610.2. BT168FO cells were processed through the MAGnify Chromatin Immunoprecipitation System (Thermo Fisher). Libraries were prepared at the Istituto di Genomica Applicata (IGA) in Udine (http://www.appliedgenomics.org/) according to Illumina TruSeq DNA Sample Preparation Guide

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

38721189

Reads aligned (%)

95.7

Duplicates removed (%)

64.8

Number of peaks

327 (qval < 1E-05)

hg19

Number of total reads

38721189

Reads aligned (%)

95.3

Duplicates removed (%)

65.5

Number of peaks

294 (qval < 1E-05)