Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AR

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC derived neural cells
NA
NA

Attributes by original data submitter

Sample

source_name
Neural Stem Cells
cell type
ES-derived neural stem cells
treatment
DHT 10nM 4 hours
antibody
AR (N-20 Santa Cruz sc-816)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cellular lysates were isolated by incubating cells pellets in SDS lysis buffer, sonicated and AR-DNA complexes were isolated with antibody. Libraries were prepared using NEXTflex ChIP-Seq Kit (#5143-02, Bioo Scientific) following the manufacturer's protocol (V12.10)

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
42587055
Reads aligned (%)
56.4
Duplicates removed (%)
41.7
Number of peaks
1141 (qval < 1E-05)

hg38

Number of total reads
42587055
Reads aligned (%)
58.4
Duplicates removed (%)
40.1
Number of peaks
1068 (qval < 1E-05)

Base call quality data from DBCLS SRA