Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AR

Cell type

Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
LNCaP
cell type
prostate cancer cell line
treatment
DHT 100nM 4 hours
antibody
AR (3279.4 homemade)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cellular lysates were isolated by incubating cells pellets in SDS lysis buffer, sonicated and AR-DNA complexes were isolated with antibodies. Libraries were prepared using NEXTflex ChIP-Seq Kit (#5143-02, Bioo Scientific) following the manufacturer's protocol (V12.10)

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
51059888
Reads aligned (%)
12.9
Duplicates removed (%)
85.8
Number of peaks
501 (qval < 1E-05)

hg38

Number of total reads
51059888
Reads aligned (%)
14.8
Duplicates removed (%)
83.9
Number of peaks
496 (qval < 1E-05)

Base call quality data from DBCLS SRA