GSM2302968: LnCap DHT 100nM IP AR 3279.4; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
AR
Cell type
Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
LNCaP
cell type
prostate cancer cell line
treatment
DHT 100nM 4 hours
antibody
AR (3279.4 homemade)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cellular lysates were isolated by incubating cells pellets in SDS lysis buffer, sonicated and AR-DNA complexes were isolated with antibodies. Libraries were prepared using NEXTflex ChIP-Seq Kit (#5143-02, Bioo Scientific) following the manufacturer's protocol (V12.10)