Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
IRF1

Cell type

Cell type Class
Blood
Cell type
Monocytes-CD14+
Tissue
monocytes
Lineage
mesoderm
Description
Monocytes-CD14+ are CD14-positive cells from human leukapheresis production, from donor RO 01746 (draw 1 ID is RO 01746, draw 2 ID is RO 01826), newly promoted to tier 2: not in 2011 analysis

Attributes by original data submitter

Sample

source_name
Peripheral blood cells
cell type
CD14+ monocytes
chip antibody
IRF1 (Santa Cruz sc-497)
experimental condition
Cultured with MCSF (10ng/ml) and IFN-gamma (100U/ml) for 24 hours, stimulated with LPS (50ng/ml) for 6 hours.

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
235412978
Reads aligned (%)
94.5
Duplicates removed (%)
86.1
Number of peaks
11777 (qval < 1E-05)

hg19

Number of total reads
235412978
Reads aligned (%)
93.4
Duplicates removed (%)
87.6
Number of peaks
11205 (qval < 1E-05)

Base call quality data from DBCLS SRA