Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

Monocytes-CD14+

Tissue

monocytes

Lineage

mesoderm

Description

Monocytes-CD14+ are CD14-positive cells from human leukapheresis production, from donor RO 01746 (draw 1 ID is RO 01746, draw 2 ID is RO 01826), newly promoted to tier 2: not in 2011 analysis

Sample

source_name

Peripheral blood cells

cell type

CD14+ monocytes

chip antibody

H3K27ac (Abcam ab-4729)

experimental condition

Cultured with MCSF (10ng/ml)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

226673757

Reads aligned (%)

96.2

Duplicates removed (%)

40.4

Number of peaks

113640 (qval < 1E-05)

hg19

Number of total reads

226673757

Reads aligned (%)

95.9

Duplicates removed (%)

40.6

Number of peaks

113304 (qval < 1E-05)