Antigen

Antigen Class

TFs and others

Antigen

STAT1

Cell type

Cell type Class

Blood

Cell type

Monocytes-CD14+

Tissue

monocytes

Lineage

mesoderm

Description

Monocytes-CD14+ are CD14-positive cells from human leukapheresis production, from donor RO 01746 (draw 1 ID is RO 01746, draw 2 ID is RO 01826), newly promoted to tier 2: not in 2011 analysis

Sample

source_name

Peripheral blood cells

cell type

CD14+ monocytes

chip antibody

STAT1 (Santa Cruz sc-345)

experimental condition

Cultured with MCSF (10ng/ml) and IFN-gamma (100U/ml) for 24 hours.

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

198062447

Reads aligned (%)

93.6

Duplicates removed (%)

20.7

Number of peaks

63959 (qval < 1E-05)

hg19

Number of total reads

198062447

Reads aligned (%)

92.9

Duplicates removed (%)

21.8

Number of peaks

63263 (qval < 1E-05)