GSM2300784: yw embr AB+ 2; Drosophila melanogaster; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27me3
Cell type
Cell type Class
Embryo
Cell type
0-4h embryos
NA
NA
Attributes by original data submitter
Sample
source_name
embryos
genotype
yw
chip antibody
Abcam anti-H3K27me3 (6002)
tissue
0-4h embryos
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
20 ul of 0-4h embryos were used as starting material. Abcam anti-H3K27me3 antibodies (6002) were used for chromatin immunoprecipitation. Chromatin was sheared using BioRuptor instrument (Diagenode). DNA from precipitated material and input were used for library preparation using Illumina Nextera protocol.